In-Vitro Hepatoprotection Study, Cytotoxicity assay and Chromatographic Investigation of Phospholipids Fraction Isolated from Cyperus esculentus Tubers Growing in Egypt

The phospholipids content of Cyperus esculentus tubers were identified by both HPTLC and/or HPLC techniques. The identified phospholipid classes were Phosphatidic acids, Phosphatidylinositol, Phosphatidylethanolamines, Phosphatidylcholine, L-α Phosphatidyl DL-glycerol, Cardiolipin, and L-α-Lysophosphatidylcholine. The hydrolysis of total phospholipids showed that four fatty acids were attached to the phospholipid classes, and identified by GC/MS as Palmitic acid (C16:0), Linoleic acid (C18:2), Oleic acid (C18:1) and Lignoceric acid (C24:0). The major fatty acids were Palmitic and Oleic acids. Phosphatidylinositol (C16:0, 18:1) was separated and identified by using HPTLC, HPLC and MALDI techniques. In vitro method of rat hepatocytes monolayer culture was used for the investigation of hepatoprotective and hepatotoxic effect of phospholipids fraction prepared from C.esculentus tubers, which were 25 and <1000 μgmL respectively. In addition, the phospholipids fraction did not show any cytotoxic effect on both U937 and PBM cells untill a concentration of 30 μgmL.